Table 2 Primers of ALDOA, CDC20, CDC45 and CCNB2 for qRT-PCR. Primer Premier 5.0 was used to design the primers, and Primer-BLAST tool of NCBI was used to ensure the accuracy and specificity of these primers
| Targeted Gene | Primer | Sequence (5′–3′) |
|---|---|---|
| ALDOA | Forward | ATGCCCTACCAATATCCAGC |
| Reverse | GACAGCCCATCCAACCCT | |
| CDC20 | Forward | GGCACCAGTGATCGACACATTCGCAT |
| Reverse | GCCATAGCCTCAGGGTCTCATCTGCT | |
| CCNB2 | Forward | GCGTTGGCATTATGGATCG |
| Reverse | TCTTCCGGGAAACTGGCTG | |
| CDC45 | Forward | TGGACTGCACACGGATCT |
| Reverse | AACCTGGCTGCGGTATAG | |
| TK1 | Forward | TGGCTGTCATAGGCATCGAC |
| Reverse | CCAGTGCAGCCACAATTACG | |
| β-Actin | Forward | AGCGAGCATCCCCCAAAGTT |
| Reverse | GGGCACGAAGGCTCATCATT |